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Igfbp5 ELISA Kit (Mouse)

CAT: 0247-OKEH04579-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04579-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Insulin-like growth factor binding protein 5
Gene Aliases
AI256729; AW208790; IBP-5; IGF-binding protein 5; IGFBP; IGFBP-5; IGFBP-5P; insulin-like growth factor-binding protein 5.
Gene ID
16011
Accession Number
NP_034648.2
Reactivity
Mouse, Mus musculus
Target
Insulin-like growth factor-binding protein 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Igfbp5 ELISA Kit (Mouse) (OKEH04579) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Igfbp5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Igfbp5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Igfbp5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Sensitivity
0.312 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
30 KDa
Specificity
Natural and recombinant Mouse Insulin-like growth factor-binding protein 5
Protein Length
271
NCBI Gene Symbol
Igfbp5
Protein Name
Q07079
Gene Name URL
Igfbp5
Nucleotide Accession Number
NM_010518.2

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