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MYC ELISA Kit (Human)

CAT: 0247-OKEH04573-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04573-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
MYC proto-oncogene, bHLH transcription factor
Gene Aliases
Avian myelocytomatosis viral oncogene homolog; bHLHe39; class E basic helix-loop-helix protein 39; c-Myc; MRTL; myc proto-oncogene protein; MYCC; myc-related translation/localization regulatory factor; proto-oncogene c-Myc; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog.
Gene ID
4609
Accession Number
NP_002458.2
Reactivity
Homo sapiens, Human
Target
Myc proto-oncogene protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MYC ELISA Kit (Human) (OKEH04573) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for MYC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYC captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Sensitivity
9.4 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
49 KDa
Specificity
Natural and recombinant Human Myc proto-oncogene protein
Protein Length
439
NCBI Gene Symbol
MYC
Protein Name
P01106
Gene Name URL
MYC
Nucleotide Accession Number
NM_002467.4

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