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TPO ELISA Kit (Human)

CAT: 0247-OKEH04556-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04556-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Thyroid peroxidase
Gene Aliases
MSA; TDH2A; thyroid microsomal antigen; thyroid peroxidase; thyroperoxidase; TPX.
Gene ID
7173
Accession Number
NP_000538.3
Reactivity
Homo sapiens, Human
Target
Thyroid peroxidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TPO ELISA Kit (Human) (OKEH04556) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TPO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for TPO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TPO captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/ml
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
103 KDa
Specificity
Natural and recombinant Human Thyroid peroxidase
Protein Length
993
NCBI Gene Symbol
TPO
Protein Name
P07202
Gene Name URL
TPO
Nucleotide Accession Number
NM_000547.5

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