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A1BG ELISA Kit (Human)

CAT: 0247-OKEH04435-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04435-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Alpha-1-B glycoprotein
Gene Aliases
A1B; ABG; Alpha-1-B glycoprotein; alpha-1B-glycoprotein; epididymis secretory sperm binding protein Li 163pA; GAB; HEL-S-163pA; HYST2477.
Gene ID
1
Accession Number
NP_570602.2
Reactivity
Homo sapiens, Human
Target
Alpha-1B-glycoprotein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology A1BG ELISA Kit (Human) (OKEH04435) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for A1BG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for A1BG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample A1BG captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
0.32 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
54 KDa
Specificity
Natural and recombinant Human Alpha-1B-glycoprotein
Protein Length
495
NCBI Gene Symbol
A1BG
Protein Name
P04217
Gene Name URL
A1BG
Nucleotide Accession Number
NM_130786.3

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