CD8A ELISA Kit (Rat)

• Gene Name:

  CD8a molecule

• Gene Aliases:

  CD8 antigen 32 kDa chain; CD8 antigen, alpha-chain; OX-8 membrane antigen; T-cell surface glycoprotein CD8 alpha chain.

• Gene ID:

  24930

• Accession Number:

  NP_113726.1

• Reactivity:

  Rat, Rattus norvegicus

• Target:

  T-cell surface glycoprotein CD8 alpha chain

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Cd8a ELISA Kit (Rat) (OKEH04392) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd8a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Cd8a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd8a captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  0.08 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  26 KDa

• Specificity:

  Natural and recombinant Rat T-cell surface glycoprotein CD8 alpha chain

• Protein Length:

  236

• NCBI Gene Symbol:

  Cd8a

• Protein Name:

  P07725

• Gene Name URL:

  Cd8a

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_031538.2