CD8A ELISA Kit (Rat)

CAT: 0247-OKEH04392-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04392-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
CD8a molecule
Gene Aliases
CD8 antigen 32 kDa chain; CD8 antigen, alpha-chain; OX-8 membrane antigen; T-cell surface glycoprotein CD8 alpha chain.
Gene ID
24930
Accession Number
NP_113726.1
Reactivity
Rat, Rattus norvegicus
Target
T-cell surface glycoprotein CD8 alpha chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cd8a ELISA Kit (Rat) (OKEH04392) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd8a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Cd8a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd8a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.08 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 KDa
Specificity
Natural and recombinant Rat T-cell surface glycoprotein CD8 alpha chain
Protein Length
236
NCBI Gene Symbol
Cd8a
Protein Name
P07725
Gene Name URL
Cd8a
Nucleotide Accession Number
NM_031538.2

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