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CD8A ELISA Kit (Dog)

CAT: 0247-OKEH03973-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03973-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
CD8a molecule
Gene Aliases
CD8 antigen, alpha polypeptide (p32) ; T-cell surface glycoprotein CD8 alpha chain.
Gene ID
403157
Accession Number
NP_001002935.1
Reactivity
Canis lupus, Dog
Target
T-cell surface glycoprotein CD8 alpha chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD8A ELISA Kit (Dog) (OKEH03973) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD8A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD8A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD8A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Sensitivity
3.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
239 kDa
Specificity
Natural and recombinant Dog T-cell surface glycoprotein CD8 alpha chain
Protein Length
239
NCBI Gene Symbol
CD8A
Protein Name
P33706
Gene Name URL
CD8A
Nucleotide Accession Number
NM_001002935.2

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