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PDE4B ELISA Kit (Human)

CAT: 0247-OKEH04320-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04320-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
_x005F Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Phosphodiesterase 4B
Gene Aliases
CAMP-specific 3',5'-cyclic phosphodiesterase 4B; DPDE4; dunce-like phosphodiesterase E4; PDE32; PDEIVB; phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila) .
Gene ID
5142
Accession Number
NP_001032416.1
Reactivity
Homo sapiens, Human
Target
CAMP-specific 3',5'-cyclic phosphodiesterase 4B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PDE4B ELISA Kit (Human) (OKEH04320) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDE4B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDE4B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDE4B captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
83 kDa
Specificity
Natural and recombinant Human cAMP-specific 3',5'-cyclic phosphodiesterase 4B
Protein Length
736
NCBI Gene Symbol
PDE4B
Protein Name
Q07343
Gene Name URL
PDE4B
Nucleotide Accession Number
NM_001037339.2

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