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PDE4B ELISA Kit (Rat)

CAT: 0247-OKCD08820-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD08820-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Phosphodiesterase 4B, cAMP Specific (PDE4B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Phosphodiesterase 4B, cAMP Specific (PDE4B) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Phosphodiesterase 4B
Gene Aliases
3',5'-cyclic AMP phosphodiesterase; cAMP-specific 3',5'-cyclic phosphodiesterase 4B; DPDE4; PDE4/IVb; Pde4b_v1; Pde4b_v2; Pde4b_v3; Pde4b_v4; Pde4B1; Pde4B2; Pde4B3; Pde4B4; Pde4b5; phosphodiesterase 4B, cAMP specific; Phosphodiesterase 4B, cAMP-specific (dunce; phosphodiesterase 4B, variant 1; phosphodiesterase 4B, variant 2; phosphodiesterase 4B, variant 3; phosphodiesterase 4B, variant 4; phosphodiesterase type 4B5.
Gene ID
24626
Accession Number
NP_058727.2
Reactivity
Rat|Rattus norvegicus
Target
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 4B, cAMP Specific (PDE4B) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 4B, cAMP Specific (PDE4B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 4B, cAMP Specific (PDE4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphodiesterase 4B, cAMP Specific (PDE4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.065ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount PDE4B Microplate 96 Wells (12 x 8 Well strips) PDE4B Lyophilized Standard 2 100X PDE4B HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount PDE4B Microplate 96 Wells (12 x 8 Well strips) PDE4B Microplate 96 Wells (12 x 8 Well strips) PDE4B Lyophilized Standard 2 PDE4B Lyophilized Standard 2 100X PDE4B HRP-Detector Anitbody 1 x 120 uL 100X PDE4B HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
Pde4b
Protein Name
CAMP-specific 3',5'-cyclic phosphodiesterase 4B
Gene Name URL
Pde4b
Nucleotide Accession Number
NM_017031.2

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