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NCOR2 ELISA Kit (Human)

CAT: 0247-OKEH04298-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04298-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Nuclear receptor corepressor 2
Gene Aliases
CTG repeat protein 26; CTG26; N-CoR2; nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; SMAP270; SMRT; SMRTE; SMRTE-tau; T3 receptor-associating factor; thyroid-, retinoic-acid-receptor-associated corepressor; TNRC14; TRAC; TRAC1; TRAC-1.
Gene ID
9612
Accession Number
NP_001070729.2
Reactivity
Homo sapiens, Human
Target
Nuclear receptor corepressor 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NCOR2 ELISA Kit (Human) (OKEH04298) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NCOR2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NCOR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NCOR2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
125-8000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
100 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
275 kDa
Specificity
Natural and recombinant Human Nuclear receptor corepressor 2
Protein Length
2525
NCBI Gene Symbol
NCOR2
Protein Name
Q9Y618
Gene Name URL
NCOR2
Nucleotide Accession Number
NM_001077261.3

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