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S1PR4 ELISA Kit (Human)

CAT: 0247-OKEH04292-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04292-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Sphingosine-1-phosphate receptor 4
Gene Aliases
EDG6; endothelial differentiation G-protein coupled receptor 6; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 6; LPC1; S1P receptor 4; S1P receptor Edg-6; S1P4; SLP4; sphingosine 1-phosphate receptor 4; sphingosine 1-phosphate receptor Edg-6.
Gene ID
8698
Accession Number
NP_003766.1
Reactivity
Homo sapiens, Human
Target
Sphingosine 1-phosphate receptor 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology S1PR4 ELISA Kit (Human) (OKEH04292) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S1PR4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S1PR4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S1PR4 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
125-8000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
89 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Human Sphingosine 1-phosphate receptor 4
Protein Length
384
NCBI Gene Symbol
S1PR4
Protein Name
O95977
Gene Name URL
S1PR4
Nucleotide Accession Number
NM_003775.3

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