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SLC40A1 ELISA Kit (Mouse)

CAT: 0247-OKEH04277-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04277-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Solute carrier family 40 (iron-regulated transporter), member 1
Gene Aliases
Duodenal-specific; Dus; Dusg; ferroportin 1; ferroportin1; Ferroportin-1; FP; Fpn1; IRE; IREG1; iron-regulated transporter 1; metal transporter protein 1; metal transporting protein 1; MTP; MTP1; O; Ol5; Pc; Pcm; polycythaemia; Slc1; Slc11a3; SLC11A3 iron transporter; Slc39a1; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3; solute carrier family 39 (iron-regulated transporter), member 1; solute carrier family 40 member 1.
Gene ID
53945
Accession Number
NP_058613.2
Reactivity
Mouse, Mus musculus
Target
Solute carrier family 40 member 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Slc40a1 ELISA Kit (Mouse) (OKEH04277) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc40a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc40a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc40a1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
0.18 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
63 kDa
Specificity
Natural and recombinant Mouse Solute carrier family 40 member 1
Protein Length
570
NCBI Gene Symbol
Slc40a1
Protein Name
Q9JHI9
Gene Name URL
Slc40a1
Nucleotide Accession Number
NM_016917.2

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