GBA3 ELISA Kit (Human)

Mean Inter-assay CV%:

7732-18-5

Glucosylceramidase beta 3 (gene/pseudogene)

CBG; CBGL1; cytosolic beta-glucosidase; cytosolic beta-glucosidase-like protein 1; cytosolic GCase; cytosolic glycosylceramidase; GLUC; glucosidase beta acid 3; glucosidase, beta, acid 3 (cytosolic) ; klotho-related protein; KLRP.

57733

NP_001121904.1

Homo sapiens, Human

Cytosolic beta-glucosidase

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology GBA3 ELISA Kit (Human) (OKEH04251) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GBA3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GBA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GBA3 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

0.156-10ng/mL

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

0.1 ng/mL

Store as indicated in product manual.

54 kDa

Natural and recombinant Human Cytosolic beta-glucosidase

469

GBA3

Q9H227

GBA3