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HSPE1 ELISA Kit (Mouse)

CAT: 0247-OKEH04194-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04194-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Heat shock protein 1 (chaperonin 10)
Gene Aliases
10 kDa chaperonin;10 kDa heat shock protein, mitochondrial;10kDa; chaperonin 10; CPN10; heat shock 10 kDa protein 1 (chaperonin 10) ; Hsp; Hsp10; mitochondrial chaperonin 10; mt-cpn10.
Gene ID
15528
Accession Number
NP_032329.1
Reactivity
Mouse, Mus musculus
Target
10 kDa heat shock protein, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hspe1 ELISA Kit (Mouse) (OKEH04194) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspe1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hspe1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hspe1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
7.8-500ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
Sensitivity
4.07 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
11 kDa
Specificity
Natural and recombinant Mouse 10 kDa heat shock protein, mitochondrial
Protein Length
102
NCBI Gene Symbol
Hspe1
Protein Name
Q64433
Gene Name URL
Hspe1
Nucleotide Accession Number
NM_008303.4

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