MMP2 ELISA Kit (Mouse)

Mean Inter-assay CV%:

7732-18-5

Matrix metallopeptidase 2

72 kDa gelatinase;72 kDa type IV collagenase;72kD gelatinase;72kD type IV collagenase;72kDa gelatinase;72kDa type IV collagenase; Clg; Clg4a; Ge; GelA; gelatinase A; matrix metalloproteinase 2; Matrix metalloproteinase-2; MMP-; MMP-2.

17390

NP_032636.1

Mouse, Mus musculus

72 kDa type IV collagenase

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology Mmp2 ELISA Kit (Mouse) (OKEH04050) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mmp2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mmp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mmp2 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

0.312-20ng/mL

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

0.167 ng/mL

Store as indicated in product manual.

74 kDa

Natural and recombinant Mouse 72 kDa type IV collagenase

662

Mmp2

P33434

Mmp2