POMC ELISA Kit (Pig)

• Gene Name:

  Proopiomelanocortin

• Gene Aliases:

  Corticotropin-lipotropin; pro-opiomelanocortin; proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin) .

• Gene ID:

  396863

• Accession Number:

  NP_999023.1

• Reactivity:

  Porcine, Sus scrofa

• Target:

  Pro-opiomelanocortin

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle:

  Aviva Systems Biology POMC ELISA Kit (Pig) (OKEH03921) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human POMC antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated POMC and incubated. The POMC found in the sample or standards competes with the biotinylated POMC for limited binding sites on the immobilized anti-human POMC antibody. Excess unbound biotinylated POMC and sample or standard POMC is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated POMC captured in the well and inversely proportional to the amount of POMC which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  0.78-50ng/ml

• Sensitivity:

  0.29 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  267 kDa

• Specificity:

  Natural and recombinant Pig Pro-opiomelanocortin

• Protein Length:

  267

• NCBI Gene Symbol:

  POMC

• Protein Name:

  P01192

• Gene Name URL:

  POMC

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_213858.1