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POMC ELISA Kit (Mouse)

CAT: 0247-OKEH03680-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03680-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Pro-opiomelanocortin-alpha
Gene Aliases
ACT; ACTH; adrenal corticotropic hormone; adrenocorticotropic hormone; alp; alph; alpha-melanocyte stimulating hormone; alphaMSH; alpha-MSH; BE; beta-endorphin; Beta-LPH; beta-M; beta-MSH; Clip; corticotropin-like intermediary peptide; corticotropin-lipotropin; gamma-; Gamma-LPH; gamma-MSH; melanotropin gamma; met-enkephalin; Npp; PO; Pomc1; Pomc-1; pro-opiomelanocortin.
Gene ID
18976
Accession Number
NP_001265510.1
Reactivity
Mouse, Mus musculus
Target
Pro-opiomelanocortin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology POMC ELISA Kit (Mouse) (OKEH03680) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Pomc antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pomc and incubated. The Pomc found in the sample or standards competes with the biotinylated Pomc for limited binding sites on the immobilized anti-human Pomc antibody. Excess unbound biotinylated Pomc and sample or standard Pomc is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pomc captured in the well and inversely proportional to the amount of Pomc which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
27 kDa
Specificity
Natural and recombinant Mouse Pro-opiomelanocortin
Protein Length
235
NCBI Gene Symbol
Pomc
Protein Name
P01193
Gene Name URL
Pomc
Nucleotide Accession Number
NM_001278581.1

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