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POMC ELISA Kit (Rat)

CAT: 0247-OKCD02777-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02777-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Proopiomelanocortin (POMC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Proopiomelanocortin (POMC) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Proopiomelanocortin
Gene Aliases
Corticotropin-lipotropin.
Gene ID
24664
Accession Number
XP_017449522.1
Reactivity
Rat|Rattus norvegicus
Target
Corticotropin: Stimulates the adrenal glands to release cortisol.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Proopiomelanocortin (POMC) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Proopiomelanocortin (POMC) and unlabeled Proopiomelanocortin (POMC) (Standards or samples) with the pre-coated antibody specific to Proopiomelanocortin (POMC) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Proopiomelanocortin (POMC) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Proopiomelanocortin (POMC) in the sample.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
123.5-10,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 102 EDTA Plasma (n=5) 99-105 102 Heparin Plasma (n=5) 93-105 99 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 102 Serum (n=5) 98-105 102 EDTA Plasma (n=5) 99-105 102 EDTA Plasma (n=5) 99-105 102 Heparin Plasma (n=5) 93-105 99 Heparin Plasma (n=5) 93-105 99
Sensitivity
< 52.5 pg/mL
Reconstitution
2°C to 8°C|-20°C
Molecular Weight
235 kDa
Components
Component Amount Anti-Pomc Microplate 96 Wells (12 x 8 Well strips) Pomc Lyophilized Standard 2 x 10 ng 100X Pomc Biotin Complex 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-Pomc Microplate 96 Wells (12 x 8 Well strips) Anti-Pomc Microplate 96 Wells (12 x 8 Well strips) Pomc Lyophilized Standard 2 x 10 ng Pomc Lyophilized Standard 2 x 10 ng 100X Pomc Biotin Complex 1 x 120 uL 100X Pomc Biotin Complex 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Proopiomelanocortin (POMC) . No significant cross-reactivity or interference between Proopiomelanocortin (POMC) and analogues was observed.
NCBI Gene Symbol
POMC
Protein Name
Pro-opiomelanocortin
Gene Name URL
POMC
Nucleotide Accession Number
XM_017594033.1

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