PTRF ELISA Kit (Mouse)

CAT: 0247-OKEH03691-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH03691-96WSize:96 Tests
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Caveolae associated 1
Gene Aliases
2310075E07Rik; AW546441; C; cav; caveolae-associated protein 1; Cavin; cavin-1; Cav-p60; polymerase I and transcript release factor; Pt; Ptrf.
Gene ID
19285
Accession Number
NP_033012.1
Reactivity
Mouse, Mus musculus
Target
Caveolae-associated protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PTRF ELISA Kit (Mouse) (OKEH03691) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ptrf has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ptrf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ptrf captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
44 kDa
Specificity
Natural and recombinant Mouse Polymerase I and transcript release factor
Protein Length
392
NCBI Gene Symbol
Cavin1
Protein Name
O54724
Gene Name URL
Cavin1
Nucleotide Accession Number
NM_008986.2

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