PTRF ELISA Kit (Human)

CAT: 0247-OKEH03516-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH03516-96WSize:96 Tests
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Caveolae associated protein 1
Gene Aliases
Caveolae-associated protein 1; CAVIN; cavin-1; CGL4; congenital generalized lipodystrophy 4; FKSG13; polymerase I and transcript release factor; PTRF; RNA polymerase I and transcript release factor; TTF-I interacting peptide 12.
Gene ID
284119
Accession Number
NP_036364.2
Reactivity
Homo sapiens, Human
Target
Caveolae-associated protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PTRF ELISA Kit (Human) (OKEH03516) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PTRF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for PTRF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PTRF captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
40.2 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
43 kDa
Specificity
Natural and recombinant Human Polymerase I and transcript release factor
Protein Length
390
NCBI Gene Symbol
CAVIN1
Protein Name
Q6NZI2
Gene Name URL
CAVIN1
Nucleotide Accession Number
NM_012232.5

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