MAP2K7 ELISA Kit (Mouse)

CAT: 0247-OKEH03686-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH03686-96WSize:96 Tests
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Mitogen-activated protein kinase kinase 7
Gene Aliases
5930412N11Rik; c-Jun N-terminal kinase kinase 2; dual specificity mitogen-activated protein kinase kinase 7; JNK kinase 2; JNK-activating kinase 2; Jnkk; JNKK 2; Jnkk2; MAP kinase kinase 7; MAPK/ERK kinase 7; MAPKK 7; Mapkk7; MEK 7; Mek7; Mkk7; Prkm; Prkmk7; protein kinase, mitogen activated, kinase 7; sek; sek2.
Gene ID
26400
Accession Number
NP_001036022.1
Reactivity
Mouse, Mus musculus
Target
Dual specificity mitogen-activated protein kinase kinase 7
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAP2K7 ELISA Kit (Mouse) (OKEH03686) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Map2k7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Map2k7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Map2k7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
59 kDa
Specificity
Natural and recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 7
Protein Length
535
NCBI Gene Symbol
Map2k7
Protein Name
Q8CE90
Gene Name URL
Map2k7
Nucleotide Accession Number
NM_001042557.2

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