LEAP2 ELISA Kit (Mouse)

Mean Inter-assay CV%:

7732-18-5

Liver-expressed antimicrobial peptide 2

Leap-2; liver-expressed antimicrobial peptide 2.

259301

NP_694709.1

Mouse, Mus musculus

Liver-expressed antimicrobial peptide 2

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Aviva Systems Biology LEAP2 ELISA Kit (Mouse) (OKEH03674) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Leap2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Leap2 and incubated. The Leap2 found in the sample or standards competes with the biotinylated Leap2 for limited binding sites on the immobilized anti-human Leap2 antibody. Excess unbound biotinylated Leap2 and sample or standard Leap2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Leap2 captured in the well and inversely proportional to the amount of Leap2 which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

0.625-40ng/ml

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

0.329 ng/mL

Store as indicated in product manual.

9 kDa

Formerly GWB-M94K70

Natural and recombinant Mouse Liver-expressed antimicrobial peptide 2

76

Leap2

Q91V13

Leap2