LEAP2 ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-assay CV%:

• Gene Name:

  Liver-expressed antimicrobial peptide 2

• Gene Aliases:

  Leap-2; liver-expressed antimicrobial peptide 2.

• Gene ID:

  259301

• Accession Number:

  NP_694709.1

• Reactivity:

  Mouse, Mus musculus

• Target:

  Liver-expressed antimicrobial peptide 2

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle:

  Aviva Systems Biology LEAP2 ELISA Kit (Mouse) (OKEH03674) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Leap2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Leap2 and incubated. The Leap2 found in the sample or standards competes with the biotinylated Leap2 for limited binding sites on the immobilized anti-human Leap2 antibody. Excess unbound biotinylated Leap2 and sample or standard Leap2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Leap2 captured in the well and inversely proportional to the amount of Leap2 which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  0.625-40ng/ml

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

• Sensitivity:

  0.329 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  9 kDa

• Notes:

  Formerly GWB-M94K70

• Specificity:

  Natural and recombinant Mouse Liver-expressed antimicrobial peptide 2

• Protein Length:

  76

• NCBI Gene Symbol:

  Leap2

• Protein Name:

  Q91V13

• Gene Name URL:

  Leap2

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_153069.3