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LY6C1 ELISA Kit (Mouse)

CAT: 0247-OKEH03615-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03615-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Lymphocyte antigen 6 complex, locus C1
Gene Aliases
AA682074; AA959465; Ly6; Ly-6; Ly6c; Ly-6C; Ly-6C1; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C.
Gene ID
17067
Accession Number
NP_001238984.1
Reactivity
Mouse, Mus musculus
Target
Lymphocyte antigen 6C1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LY6C1 ELISA Kit (Mouse) (OKEH03615) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ly6c1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ly6c1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ly6c1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
Sensitivity
0.42 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
14 kDa
Specificity
Natural and recombinant Mouse Lymphocyte antigen 6C1
Protein Length
131
NCBI Gene Symbol
Ly6c1
Protein Name
P0CW02
Gene Name URL
Ly6c1
Nucleotide Accession Number
NM_001252055.1

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