DKK2 ELISA Kit (Mouse)

CAT: 0247-OKEH03567-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH03567-96WSize:96 Tests
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Dickkopf WNT signaling pathway inhibitor 2
Gene Aliases
Dickkopf 2; dickkopf homolog 1; dickkopf homolog 2; dickkopf-related protein 2; dkk-2; mDkk-2.
Gene ID
56811
Accession Number
NP_064661.2
Reactivity
Mouse, Mus musculus
Target
Dickkopf-related protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DKK2 ELISA Kit (Mouse) (OKEH03567) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dkk2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Dkk2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dkk2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
7.94 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Mouse Dickkopf-related protein 2
Protein Length
259
NCBI Gene Symbol
Dkk2
Protein Name
Q9QYZ8
Gene Name URL
Dkk2
Nucleotide Accession Number
NM_020265.4

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