DCK ELISA Kit (Human)

Mean Inter-assay CV%:

7732-18-5

Deoxycytidine kinase

Deoxyadenosine kinase; deoxycytidine kinase; deoxyguanosine kinase; deoxynucleoside kinase.

1633

NP_000779.1

Homo sapiens, Human

Deoxycytidine kinase

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology DCK ELISA Kit (Human) (OKEH03563) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DCK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for DCK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DCK captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

0.156-10ng/mL

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%

0.083 ng/mL

Store as indicated in product manual.

31 kDa

Natural and recombinant Human Deoxycytidine kinase

260

DCK

P27707

DCK