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PRL8A8 ELISA Kit (Mouse)

CAT: 0247-OKEH03560-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03560-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Prolactin family 8, subfamily a, member 81
Gene Aliases
1600032B14Rik; Ghd14; growth hormone d14; placental prolactin-like protein C3; PLP-C; PLP-C gamma2; PLP-C3; PLP-Cgamma; Pr; PRL-like protein C3; Prlpc3; prolactin-8A8; prolactin-like protein C-gamma.
Gene ID
74188
Accession Number
NP_001298054.1
Reactivity
Mouse, Mus musculus
Target
Prolactin-8A8
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRL8A8 ELISA Kit (Mouse) (OKEH03560) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Prl8a8 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Prl8a8 and incubated. The Prl8a8 found in the sample or standards competes with the biotinylated Prl8a8 for limited binding sites on the immobilized anti-human Prl8a8 antibody. Excess unbound biotinylated Prl8a8 and sample or standard Prl8a8 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Prl8a8 captured in the well and inversely proportional to the amount of Prl8a8 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
39.7 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Mouse Prolactin-8A8
Protein Length
241
NCBI Gene Symbol
Prl8a8
Protein Name
Q9DAS4
Gene Name URL
Prl8a8
Nucleotide Accession Number
NM_001311125.1

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