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NEIL3 ELISA Kit (Mouse)

CAT: 0247-OKEH03545-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03545-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Nei like 3 (E. coli)
Gene Aliases
AI449477; BC034753; C85903; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; endonuclease 8-like 3; endonuclease VIII-like 3; nei endonuclease VIII-like 3; nei-like protein 3.
Gene ID
234258
Accession Number
NP_666320.1
Reactivity
Mouse, Mus musculus
Target
Endonuclease 8-like 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NEIL3 ELISA Kit (Mouse) (OKEH03545) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Neil3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Neil3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Neil3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
0.418 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
67 kDa
Specificity
Natural and recombinant Mouse Endonuclease 8-like 3
Protein Length
606
NCBI Gene Symbol
Neil3
Protein Name
Q8K203
Gene Name URL
Neil3
Nucleotide Accession Number
NM_146208.2

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