TSTA3 ELISA Kit (Human)

CAT: 0247-OKEH03537-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH03537-96WSize:96 Tests
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
GDP-L-fucose synthase
Gene Aliases
3-5 epimerase/4-reductase; FX; GDP-4-keto-6-deoxy-D-mannose epimerase-reductase; GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; GDP-L-fucose synthase; P35B; Protein FX; red cell NADP (H) -binding protein; SDR4E1; short chain dehydrogenase/reductase family 4E, member 1; Short-chain dehydrogenase/reductase family 4E member 1; testis tissue sperm-binding protein Li 45a; tissue specific transplantation antigen 3; tissue specific transplantation antigen P35B; TSTA3.
Gene ID
7264
Accession Number
NP_001304712.1
Reactivity
Homo sapiens, Human
Target
GDP-L-fucose synthase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology TSTA3 ELISA Kit (Human) (OKEH03537) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human TSTA3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TSTA3 and incubated. The TSTA3 found in the sample or standards competes with the biotinylated TSTA3 for limited binding sites on the immobilized anti-human TSTA3 antibody. Excess unbound biotinylated TSTA3 and sample or standard TSTA3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TSTA3 captured in the well and inversely proportional to the amount of TSTA3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94.9%
Sensitivity
0.172 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
36 kDa
Specificity
Natural and recombinant Human GDP-L-fucose synthase
Protein Length
321
NCBI Gene Symbol
GFUS
Protein Name
Q13630
Gene Name URL
GFUS
Nucleotide Accession Number
NM_001317783.1

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