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HUWE1 ELISA Kit (Mouse)

CAT: 0247-OKEH03504-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03504-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
HECT, UBA and WWE domain containing 1
Gene Aliases
5430439H10Rik; Arf-; Arf-bp1; AU041296; C430014N20Rik; C80292; E3 ubiquitin-protein ligase HUWE1; E3Histone; Gm1718; HECT, UBA and WWE domain-containing protein 1; HECT-type E3 ubiquitin transferase HUWE1; Ib77; Ib772; LASU1; Mcl-1 ubiquitin ligase; Mul; Mule; upstream regulatory element binding protein 1; Upstream regulatory element-binding protein 1; Ureb; Ureb1; URE-B1; URE-binding protein 1.
Gene ID
59026
Accession Number
NP_067498.4
Reactivity
Mouse, Mus musculus
Target
E3 ubiquitin-protein ligase HUWE1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HUWE1 ELISA Kit (Mouse) (OKEH03504) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Huwe1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Huwe1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Huwe1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
483 kDa
Specificity
Natural and recombinant Mouse E3 ubiquitin-protein ligase HUWE1
Protein Length
4377
NCBI Gene Symbol
Huwe1
Protein Name
Q7TMY8
Gene Name URL
Huwe1
Nucleotide Accession Number
NM_021523.4

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