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SMAD2 ELISA Kit (Mouse)

CAT: 0247-OKEH03472-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03472-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
SMAD family member 2
Gene Aliases
7120426M23Rik; Mad; MAD homolog 2; Madh; Madh2; Madr2; mad-related protein 2; mMad2; mothers against decapentaplegic homolog 2; mothers against DPP homolog 2; Smad 2; SMAD family member 2; Smad-2.
Gene ID
17126
Accession Number
NP_001239410.1
Reactivity
Mouse, Mus musculus
Target
Mothers against decapentaplegic homolog 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SMAD2 ELISA Kit (Mouse) (OKEH03472) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Smad2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Smad2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Smad2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
52 kDa
Specificity
Natural and recombinant Mouse Mothers against decapentaplegic homolog 2
Protein Length
467
NCBI Gene Symbol
Smad2
Protein Name
Q62432
Gene Name URL
Smad2
Nucleotide Accession Number
NM_001252481.1

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