WNT3A ELISA Kit (Mouse)

CAT: 0247-OKEH03470-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH03470-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Wingless-type MMTV integration site family, member 3A
Gene Aliases
Protein Wnt-3a; vestigial tail; vt; wingless-related MMTV integration site 3A; Wnt-; Wnt-3a.
Gene ID
22416
Accession Number
NP_033548.1
Reactivity
Mouse, Mus musculus
Target
Protein Wnt-3a
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology WNT3A ELISA Kit (Mouse) (OKEH03470) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Wnt3a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Wnt3a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Wnt3a captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
0.168 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 kDa
Specificity
Natural and recombinant Mouse Protein Wnt-3a
Protein Length
352
NCBI Gene Symbol
Wnt3a
Protein Name
P27467
Gene Name URL
Wnt3a
Nucleotide Accession Number
NM_009522.2

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