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GHRL ELISA Kit (Rat)

CAT: 0247-OKEH03462-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03462-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Ghrelin and obestatin prepropeptide
Gene Aliases
Appetite-regulating hormone; ghrelin/obestatin prepropeptide; growth hormone secretagogue; growth hormone-releasing peptide; motilin-related peptide.
Gene ID
59301
Accession Number
NP_067701.1
Reactivity
Rat, Rattus norvegicus
Target
Appetite-regulating hormone
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GHRL ELISA Kit (Rat) (OKEH03462) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Ghrl antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ghrl and incubated. The Ghrl found in the sample or standards competes with the biotinylated Ghrl for limited binding sites on the immobilized anti-Rat Ghrl antibody. Excess unbound biotinylated Ghrl and sample or standard Ghrl is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ghrl captured in the well and inversely proportional to the amount of Ghrl which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
13 kDa
Specificity
Natural and recombinant Rat Appetite-regulating hormone
Protein Length
117
NCBI Gene Symbol
Ghrl
Protein Name
Q9QYH7
Gene Name URL
Ghrl
Nucleotide Accession Number
NM_021669.2

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