GHRL ELISA Kit (Cattle)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Ghrelin and obestatin prepropeptide

Appetite-regulating hormone; Gh1-1; ghrelin; ghrelin/obestatin preprohormone; growth hormone secretagogue; growth hormone-releasing peptide; motilin-related peptide.

281192

NP_776492.1

Bos taurus|Bovine

Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) . Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation (By similarity) .

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Colorimetric

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ghrelin (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ghrelin (GHRL) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

123.5-10,000pg/mL

Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 90-104 98 _x005F EDTA Plasma (n=5) 96-103 101 _x005F Heparin Plasma (n=5) 80-97 84 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 90-104 98 Serum (n=5) 90-104 98 EDTA Plasma (n=5) 96-103 101 EDTA Plasma (n=5) 96-103 101 Heparin Plasma (n=5) 80-97 84 Heparin Plasma (n=5) 80-97 84

< 49.2 pg/mL

2°C to 8°C|-20°C

116 kDa

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This assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL) . No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.

GHRL

Appetite-regulating hormone

GHRL