CD59A ELISA Kit (Mouse)

CAT: 0247-OKEH03456-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH03456-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
CD59a antigen
Gene Aliases
AA987121; Cd59; CD59 antigen; CD59A glycoprotein; MACIF; MAC-inhibitory protein; MAC-IP; membrane attack complex inhibition factor; prot; protectin.
Gene ID
12509
Accession Number
NP_001104530.1
Reactivity
Mouse, Mus musculus
Target
CD59A glycoprotein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD59A ELISA Kit (Mouse) (OKEH03456) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd59a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cd59a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd59a captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
14 kDa
Specificity
Natural and recombinant Mouse CD59A glycoprotein
Protein Length
123
NCBI Gene Symbol
Cd59a
Protein Name
O55186
Gene Name URL
Cd59a
Nucleotide Accession Number
NM_001111060.2

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