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GAPDH ELISA Kit (Mouse)

CAT: 0247-OKEH03426-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03426-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, pseudogene 15
Gene Aliases
Ga; Gapd; glyceraldehyde-3-phosphate dehydrogenase; Gm20899; peptidyl-cysteine S-nitrosylase GAPDH.
Gene ID
100042025, 14433
Accession Number
NP_001276655.1
Reactivity
Mouse, Mus musculus
Target
Glyceraldehyde-3-phosphate dehydrogenase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GAPDH ELISA Kit (Mouse) (OKEH03426) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gapdh has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gapdh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gapdh captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
0.18 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
36 kDa
Specificity
Natural and recombinant Mouse Glyceraldehyde-3-phosphate dehydrogenase
Protein Length
333
NCBI Gene Symbol
Gapdh, Gapdh-ps15
Protein Name
P16858
Gene Name URL
Gapdh, Gapdh-ps15
Nucleotide Accession Number
NM_001289726.1