GAPDH ELISA Kit (Human)

• Gene Name:

  Glyceraldehyde-3-phosphate dehydrogenase

• Gene Aliases:

  Aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; G3PD; GAPD; glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; peptidyl-cysteine S-nitrosylase GAPDH.

• Gene ID:

  2597

• Accession Number:

  NP_001243728.1

• Reactivity:

  Homo sapiens, Human

• Target:

  Glyceraldehyde-3-phosphate dehydrogenase

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology GAPDH ELISA Kit (Human) (OKEH01375) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GAPDH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GAPDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GAPDH captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

• Detection Range:

  0.156-10ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%

• Sensitivity:

  0.1 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  36 kDa

• Notes:

  Formerly GWB-UBRKAG

• Specificity:

  Natural and recombinant Human Glyceraldehyde-3-phosphate dehydrogenase

• Protein Length:

  335

• NCBI Gene Symbol:

  GAPDH

• Protein Name:

  P04406

• Gene Name URL:

  GAPDH

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001256799.2