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BTK ELISA Kit (Mouse)

CAT: 0247-OKEH03422-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03422-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Bruton agammaglobulinemia tyrosine kinase
Gene Aliases
Agammaglobulinaemia tyrosine kinase; agammaglobulinemia tyrosine kinase; AI528679; ATK; B-cell progenitor kinase; BPK; bruton tyrosine kinase; Bruton's tyrosine kinase; kinase EMB; tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB) ; xi; xid; X-linked immune deficiency.
Gene ID
12229
Accession Number
NP_038510.2
Reactivity
Mouse, Mus musculus
Target
Tyrosine-protein kinase BTK
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BTK ELISA Kit (Mouse) (OKEH03422) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Btk has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Btk is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Btk captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.092 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
76 kDa
Specificity
Natural and recombinant Mouse Tyrosine-protein kinase BTK
Protein Length
659
NCBI Gene Symbol
Btk
Protein Name
P35991
Gene Name URL
Btk
Nucleotide Accession Number
NM_013482.2

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