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GSTA3 ELISA Kit (Rat)

CAT: 0247-OKEH03403-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03403-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Glutathione S-transferase alpha 1, glutathione S-transferase alpha-3
Gene Aliases
Glutathione S-transferase A1; glutathione S-transferase A3 subunit; glutathione S-transferase A5; glutathione S-transferase alpha-3; Glutathione S-transferase Yc-1; glutathione S-transferase Yc1 subunit; Glutathione-S-transferase alpha type (Ya) ; GST 2-2; GST A3-3; GST AA; GST Yc1; Gsta3; Gsta5; Yc1.
Gene ID
108348061, 24421
Accession Number
NP_113697.1
Reactivity
Rat, Rattus norvegicus
Target
Glutathione S-transferase alpha-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GSTA3 ELISA Kit (Rat) (OKEH03403) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gsta3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gsta3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gsta3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.091 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
25 kDa
Specificity
Natural and recombinant Rat Glutathione S-transferase alpha-3
Protein Length
221
NCBI Gene Symbol
Gsta1, LOC108348061
Protein Name
P04904
Gene Name URL
Gsta1, LOC108348061
Nucleotide Accession Number
NM_031509.2

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