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Gsta4 ELISA Kit (Rat)

CAT: 0247-OKEH02761-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02761-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Glutathione S-transferase alpha 4
Gene Aliases
Glutathione S-transferase A4; glutathione S-transferase alpha-4; glutathione S-transferase Yk; glutathione transferase; GST 8-8; GST A4-4; GST K; GST Yk.
Gene ID
300850
Accession Number
NP_034487.2
Reactivity
Rat, Rattus norvegicus
Target
Glutathione S-transferase alpha-4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Gsta4 ELISA Kit (Rat) (OKEH02761) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Gsta4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gsta4 and incubated. The Gsta4 found in the sample or standards competes with the biotinylated Gsta4 for limited binding sites on the immobilized anti-rat Gsta4 antibody. Excess unbound biotinylated Gsta4 and sample or standard Gsta4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gsta4 captured in the well and inversely proportional to the amount of Gsta4 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
0.442 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Specificity
Natural and recombinant Rat Glutathione S-transferase alpha-4
Protein Length
222
NCBI Gene Symbol
Gsta4
Protein Name
P14942
Gene Name URL
Gsta4
Nucleotide Accession Number
NM_010357.3

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