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MAPK1 ELISA Kit (Mouse)

CAT: 0247-OKEH03316-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03316-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Mitogen-activated protein kinase 1
Gene Aliases
9030612K14Rik; AA407128; AU018647; C78273; ERK; Erk2; ERK-2; ERT1; extracellular signal-regulated kinase 2; MAP; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; MAPK2; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; p41mapk; p42m; p42mapk; p42-MAPK; Prk; Prkm1; PRKM2.
Gene ID
26413
Accession Number
NP_001033752.1
Reactivity
Mouse, Mus musculus
Target
Mitogen-activated protein kinase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAPK1 ELISA Kit (Mouse) (OKEH03316) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mapk1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Mapk1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mapk1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
41.3 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
41 kDa
Specificity
Natural and recombinant Mouse Mitogen-activated protein kinase 1
Protein Length
358
NCBI Gene Symbol
Mapk1
Protein Name
P63085
Gene Name URL
Mapk1
Nucleotide Accession Number
NM_001038663.1

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