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MTTP ELISA Kit (Mouse)

CAT: 0247-OKEH03296-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH03296-96WSize:96 Well
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Microsomal triglyceride transfer protein
Gene Aliases
1810043K16Rik; microsomal triglyceride transfer protein B; microsomal triglyceride transfer protein large subunit; microsomal triglyceride transfer protein, large subunit; MT; MTP.
Gene ID
17777
Accession Number
NP_001156929.1
Reactivity
Mouse, Mus musculus
Target
Microsomal triglyceride transfer protein large subunit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MTTP ELISA Kit (Mouse) (OKEH03296) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mttp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Mttp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mttp captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
0.167 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
99 kDa
Specificity
Natural and recombinant Mouse Microsomal triglyceride transfer protein large subunit
Protein Length
894
NCBI Gene Symbol
Mttp
Protein Name
O08601
Gene Name URL
Mttp
Nucleotide Accession Number
NM_001163457.1

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