PPARA ELISA Kit (Mouse)

CAT: 0247-OKEH03249-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH03249-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Peroxisome proliferator activated receptor alpha
Gene Aliases
4933429D07Rik; AW742785; Nr1; Nr1c1; nuclear receptor subfamily 1 group C member 1; P; peroxisome proliferator-activated receptor alpha; PP; Ppar; PPARalpha; PPAR-alpha.
Gene ID
19013
Accession Number
NP_001106889.1
Reactivity
Mouse, Mus musculus
Target
Peroxisome proliferator-activated receptor alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PPARA ELISA Kit (Mouse) (OKEH03249) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppara has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ppara is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppara captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
52 kDa
Specificity
Natural and recombinant Mouse Peroxisome proliferator-activated receptor alpha
Protein Length
468
NCBI Gene Symbol
Ppara
Protein Name
P23204
Gene Name URL
Ppara
Nucleotide Accession Number
NM_001113418.1

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