PPARA ELISA Kit (Human)

CAT: 0247-OKCA02297-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA02297-96WSize:96 Well
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
PPAR, NR1C1, hPPAR, PPARalpha, PPAR-alpha
Gene ID
5465
Accession Number
NP_001001928
Reactivity
Human
Target
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC) . Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology PPARA ELISA Kit (Human) (OKCA02297) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPARA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPARA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard PPARA captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
78 - 5,000 pg/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 89-98 EDTA Plasma (n=4) 98 90-101 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 89-98 Serum (n=5) 95 89-98 EDTA Plasma (n=4) 98 90-101 EDTA Plasma (n=4) 98 90-101
Sensitivity
19.5 pg/mL.
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
PPARA
Protein Name
Peroxisome proliferator-activated receptor alpha
Gene Name URL
PPARA
Nucleotide Accession Number
NM_001001928

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