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PTH ELISA Kit (Rat)

CAT: 0247-OKEH03237-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03237-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Parathyroid hormone
Gene Aliases
Hypothalamic parathyroid hormone; parathyrin; parathyroid hormone; PTH- (1-84) ; Pth1; Pthr1.
Gene ID
24694
Accession Number
NP_058740.1
Reactivity
Rat, Rattus norvegicus
Target
Parathyroid hormone
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PTH ELISA Kit (Rat) (OKEH03237) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pth has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pth is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pth captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
23.4-1500pg/mL
Sensitivity
11.7 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
13 kDa
Specificity
Natural and recombinant Rat Parathyroid hormone
Protein Length
115
NCBI Gene Symbol
Pth
Protein Name
P04089
Gene Name URL
Pth
Nucleotide Accession Number
NM_017044.1

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