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C1QA ELISA Kit (Mouse)

CAT: 0247-OKEH03210-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03210-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Complement component 1, q subcomponent, alpha polypeptide
Gene Aliases
Adic; adiponectin c; AI255395; C1q; complement C1q subcomponent subunit A.
Gene ID
12259
Accession Number
NP_031598.2
Reactivity
Mouse, Mus musculus
Target
Complement C1q subcomponent subunit A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology C1QA ELISA Kit (Mouse) (OKEH03210) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1qa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for C1qa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C1qa captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
7.10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Specificity
Natural and recombinant Mouse Complement C1q subcomponent subunit A
Protein Length
245
NCBI Gene Symbol
C1qa
Protein Name
P98086
Gene Name URL
C1qa
Nucleotide Accession Number
NM_007572.2

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