C1QA ELISA Kit (Rat)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement Component 1, Q Subcomponent A (C1qA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement Component 1, Q Subcomponent A (C1qA) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Complement C1q A chain

• Gene Aliases:

  Adic; adiponectin c; complement C1q subcomponent subunit A; complement component 1, q subcomponent, A chain; complement component 1, q subcomponent, alpha polypeptide.

• Gene ID:

  298566

• Accession Number:

  NP_001008515.1

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca (2+) -dependent C1r (2) C1s (2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Subcomponent A (C1qA) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, Q Subcomponent A (C1qA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, Q Subcomponent A (C1qA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Component 1, Q Subcomponent A (C1qA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  1.56-100ng/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 79-88 80 EDTA Plasma (n=5) 78-104 93 Heparin Plasma (n=5) 86-104 92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 79-88 80 Serum (n=5) 79-88 80 EDTA Plasma (n=5) 78-104 93 EDTA Plasma (n=5) 78-104 93 Heparin Plasma (n=5) 86-104 92 Heparin Plasma (n=5) 86-104 92

• Sensitivity:

  0.67 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-C1QA Microplate 96 Wells (12 x 8 Well strips) C1QA Lyophilized Standard 2 100X Biotinylated C1QA Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-C1QA Microplate 96 Wells (12 x 8 Well strips) Anti-C1QA Microplate 96 Wells (12 x 8 Well strips) C1QA Lyophilized Standard 2 C1QA Lyophilized Standard 2 100X Biotinylated C1QA Detector Antibody 1 x 120 uL 100X Biotinylated C1QA Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Complement Component 1, Q Subcomponent A (C1qA) . No significant cross-reactivity or interference between Complement Component 1, Q Subcomponent A (C1qA) and analogues was observed.

• Protein Length:

  245

• NCBI Gene Symbol:

  C1qa

• Protein Name:

  Complement C1q subcomponent subunit A

• Gene Name URL:

  C1qa

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001008515.1