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BGLAP ELISA Kit (Rat)

CAT: 0247-OKEH03127-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03127-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Bone gamma-carboxyglutamate protein
Gene Aliases
Bglap2; Bgp; Bgpr; Bgpra; bone gamma-carboxyglutamate (gla) protein (osteocalcin) ; bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin) ; Bone Gla protein; bone Gla-protein; gamma-carboxyglutamic acid-containing protein; osteocalcin.
Gene ID
25295
Accession Number
NP_038200.1
Reactivity
Rat, Rattus norvegicus
Target
Osteocalcin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BGLAP ELISA Kit (Rat) (OKEH03127) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bglap has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Bglap is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bglap captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
62.5-4000pg/mL
Recovery
Mean recovery when spiking into serum: 100%
Sensitivity
31.31 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
11 kDa
Specificity
Natural and recombinant Rat Osteocalcin
Protein Length
99
NCBI Gene Symbol
Bglap
Protein Name
P04640
Gene Name URL
Bglap
Nucleotide Accession Number
NM_013414.1

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