BGLAP ELISA Kit (Mouse)

CAT: 0247-OKEH00462-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00462-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Bone gamma carboxyglutamate protein
Gene Aliases
Bgl; Bglap1; BGP; bone gamma carboxyglutamate protein 1; bone Gla protein; gamma-carboxyglutamic acid-containing protein; mOC; mOC-A; O; OC; OG; OG1; oste; osteocalcin.
Gene ID
12096
Accession Number
NP_031567.1
Reactivity
Mouse, Mus musculus
Target
Osteocalcin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BGLAP ELISA Kit (Mouse) (OKEH00462) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse BGLAP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mouse BGLAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mouse BGLAP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/ml
Sensitivity
7.84 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
10 kDa
Specificity
Natural and recombinant Mouse Osteocalcin
Protein Length
95
NCBI Gene Symbol
Bglap
Protein Name
P86546
Gene Name URL
Bglap
Nucleotide Accession Number
NM_007541.3

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