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GPT ELISA Kit (Rat)

CAT: 0247-OKEH03060-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03060-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Glutamic--pyruvic transaminase
Gene Aliases
Alanine aminotransferase 1; ALAT; ALT1; glutamate pyruvate transaminase 1; glutamic pyruvate transaminase; glutamic pyruvic transaminase (alanine aminotrasferase) ; glutamic pyruvic transaminase 1, soluble; glutamic--alanine transaminase 1; glutamic-pyruvate transaminase (alanine aminotransferase) ; glutamic--pyruvic transaminase 1; GPT 1; Gpt1.
Gene ID
81670
Accession Number
NP_112301.1
Reactivity
Rat, Rattus norvegicus
Target
Alanine aminotransferase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GPT ELISA Kit (Rat) (OKEH03060) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpt has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Gpt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gpt captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.78-50ng/mL
Sensitivity
0.402 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
55 kDa
Specificity
Natural and recombinant Rat Alanine aminotransferase 1
Protein Length
496
NCBI Gene Symbol
Gpt
Protein Name
P25409
Gene Name URL
Gpt
Nucleotide Accession Number
NM_031039.1

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