Products for Research Use Only
RegisterLogin

GPT ELISA Kit (Mouse)

CAT: 0247-OKCD06133-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD06133-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Alanine Aminotransferase (ALT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Alanine Aminotransferase (ALT) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Glutamic pyruvic transaminase, soluble
Gene Aliases
1300007J06Rik;2310022B03Rik; A; alanine aminotransferase 1; ALT; ALT1; glutamate pyruvate transaminase 1; glutamic pyruvic transaminase 1, soluble; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; Gpt-; GPT 1; Gpt1; Gpt-1.
Gene ID
76282
Accession Number
NP_877957.1
Reactivity
Mouse|Mus musculus
Target
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase (ALT) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alanine Aminotransferase (ALT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alanine Aminotransferase (ALT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alanine Aminotransferase (ALT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Detection Range
1.56-100ng/mL
Sensitivity
< 0.064ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount GPT Microplate 96 Wells (12 x 8 Well strips) GPT Lyophilized Standard 2 100X GPT HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount GPT Microplate 96 Wells (12 x 8 Well strips) GPT Microplate 96 Wells (12 x 8 Well strips) GPT Lyophilized Standard 2 GPT Lyophilized Standard 2 100X GPT HRP-Detector Anitbody 1 x 120 uL 100X GPT HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
Gpt
Protein Name
Alanine aminotransferase 1
Gene Name URL
Gpt
Nucleotide Accession Number
NM_182805.3

Related Products

CatalogName
OKCD06132-96WGPT ELISA Kit (Human)
OKEH00300-96WGPT ELISA Kit (Pig)
OKEH00299-96WGPT ELISA Kit (Human)
OKCD06134-96WGPT ELISA Kit (Rat)
OKEH03060-96WGPT ELISA Kit (Rat)
OKCD06132GPT ELISA Kit (Human) (OKCD06132)
OKAN05442GPT ELISA Kit (Rat) (OKAN05442)
OKCD06134GPT ELISA Kit (Rat) (OKCD06134)
OKAN05433GPT ELISA Kit (Human) (OKAN05433)
OKCD06131GPT ELISA Kit (Bovine) (OKCD06131)

Alternative Products

CatalogName
GTR10374983Mouse Cholesterol ELISA ELISA
EA-2402Mouse FGFb ELISA
EA-2403Mouse EGF ELISA
EA-2203Mouse TNF-a ELISA
EA-2202Mouse Leptin ELISA
TBS3099Mouse Glucagon ELISA
E109969Mouse Aβ ELISA Kit
E062537Mouse ON ELISA Kit
1037Mouse Nephrin ELISA
YP-W22083-01Mouse AT (AT) ELISA Kit

Popular Products