IL36A ELISA Kit (Mouse)

CAT: 0247-OKEH02999-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02999-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Interleukin 36A
Gene Aliases
Fi; Fil; Fil1; FIL1 epsilon; Fil1epsilon; If36a; IL-1; IL-1 epsilon; Il1f6; IL-1F6; Il1f9; IL-1H1; IL1RP2; interleukin 1 family, member 6 (epsilon) ; interleukin 1 family, member 9; interleukin-1 epsilon; interleukin-1 family member 6; interleukin-1 homolog 1; interleukin-36 alpha.
Gene ID
54448
Accession Number
NP_062323.1
Reactivity
Mouse, Mus musculus
Target
Interleukin-36 alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IL36A ELISA Kit (Mouse) (OKEH02999) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il36a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Il36a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il36a captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/ml
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
18 kDa
Specificity
Natural and recombinant Mouse Interleukin-36 alpha
Protein Length
160
NCBI Gene Symbol
Il36a
Protein Name
Q9JLA2
Gene Name URL
Il36a
Nucleotide Accession Number
NM_019450.3

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